After finishing Gymnasium in Pardubice she studied the choirmastership of church music at Collegium Sanctissimae Mariae in Prague and orchestral conducting at the Jaroslav Ježek Conservatory. She graduated from the Jaroslav Ježek Conservatory in singing and from the Pedagogical Faculty of Charles University in choirmastership and music education.

She currently teaches at the Musical department of the Jaroslav Ježek Conservatory, Týnská School, the Pedagogical Faculty of Charles University and since 2010 she has also been an external teacher of singing at the Theatre Faculty of the Academy of Musical Arts in Prague. Pavla Fendrichová is a popular lecturer at vocal workshops (Germany, Sweden, Switzerland, CR) and a voice coach (cooperation with Czech Television and Prague theatres) of vocal and vocal-instrumental ensembles.

From 2000 to 2004 she founded and led the Ensemble Guillaume, which specializes in early music. From 2001 to 2007 she was an artistic director and a choirmaster of the Collegium Cantatium women's chamber choir, which became a laureate of many international competitions under her leadership.

As a singer, she regularly performs at home and abroad (Germany, Switzerland, Austria, Italy, the Netherlands, Poland, Hungary, and Slovakia). For example, she had a recital at the International Festival of Early Music in Polish Radom 2001, the Festival of Sacred Music Česká Lípa 2004 and ARS ANTIQUA EUROPAE 2005, the Prague Gates Festival 2007, the Haydn Music Festival 2008 and Hofer Konzerte 2010. She has studied early music at a number of masterclasses under outstanding music teachers (Nigel Rogers, Poppy Holden, Nicholas Clapton, Marius van Altena, Stephen Stubbs and others). She has collaborated with a number of significant musical personalities and bodies, among them English lutenist Brian Wright, guitarist Patrick Henel, Slovak lutenist Martin Celhoffer, and Collegium Millennium, Collegium Marianum, and Syrinx along with the Chamber Philharmonic Orchestra Pardubice, Musica Fresca, and Musa Animae. Since 2003 she has been a soprano with the legendary Linha Singers. She has made two solo CDs. The focus of her vocal work is collaboration with lutenist Jindřich Macek, with whom she has created a vocal-instrumental duo involving theoretical and practical interpretations of Renaissance and early Baroque songs with lute accompaniment. During this collaboration, which began in 2002, they have repeatedly gone to areas other than those of old music - e.g. folk music and 20th century music.

Together with Jinřichem Macek she performs in projects for voice and lute: